Technical Data
A3000-14A
Aquaporin 4 (AQP4, WCH4, MIWC, Mercury-insensitive Water Channel)
Description:
Aquaporin 4 is located in various tissues including the gastro intestinal tract, kidney and brain.

Applications:
Suitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry(frozen and paraffin): 1:50 Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
ELISA: 1:500-1:10,000
Immunoprecipitation: 1:500-1:5000
Western Blot: 1:500-1:5000
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo

Recommended Positive Control Tissue:
Kidney

Hybridoma:
Spleen cells from immunized Balb/c mice were fused with the cells of Sp-2/0 Ag14 mouse myeloma cell line.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H552Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to amino acids 301-318 of rat aquaporin 4.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid with 0.1% BSA., 0.09% sodium azide, before addition of glycerol to 40%
Specificity:
Recognizes an epitope within the cytoplasmic domain of the water-specific channel of human aquaporin 4 (AQP4). Does not react with mouse or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bodis, B. et al. (2001). Active water selective channels in the stomach: investigation of aquaporins after ethanol and capsaicin treatment in rats. Journal of Physiology. 95: 271 - 275