Technical Data
A3000-14B
Aquaporin 4 (AQP4, WCH4, MIWC, Mercury-insensitive Water Channel)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporins". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP4 (MIWC, mercury-insensitive water channel) is expressed in brain and in other tissues as well. Aquaporin 4 is located in various tissues including the gastro-intestinal tract, kidney and brain.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:2000
Western Blot: 1:1000-1:10,000; Predicted MW: 38.8kD
Immunoprecipitation: 1:1000 -1:10,000
Immunohistochemistry (paraffin): 1:100; Perform heat mediated antigen retrieval with sodium citrate buffer pH 6.0.
Immunohistochemistry (frozen): 1:100; Use snap-frozen, acetone-fixed tissue only.
Optimal dilutions to be determined by the researcher.

Positive Control:
Kidney tissue

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG18J301Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa301-318 of rat Aquaporin 4. Cellular Localization: Plasma membrane.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA., 0.09% sodium azide.
Specificity:
Recognizes an epitope within the cytoplasmic domain of water-specific channel Aquaporin 4. Species Crossreactivity: human. Does not crossreact with rat or mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Badis, B., et al., J. Physiol. Paris 95: 271-275 (2001). 2. Nagy, G., et al., Pathol. Oncol. Res. 8(2): 115-124 (2002).