Technical Data
A3000-17A
Aquaporin 4 (AQP4, WCH4, MIWC)
Description:
Rat AQP4 is a 301 amino acid transmembrane protein. It belongs to the MIP family. Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin”. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Human Aquaporin-0 is a 263 amino acid transmembrane protein belonging to the MIP family. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N- and C-terminus are predicted to be cytoplasmic.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000. Note: Recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
ELISA: 1:100,000. A3000-18: Aquaporin 4, Rat Control Peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4°C (-20°C Glycerol)Blue IceRatRabbit
Concentration:
Not Determined.
Immunogen:
Synthetic peptide 17aa sequence corresponding to aa280-296 in the region near the carboxy terminus of the rat AQP4 (1,2) (KLH coupled via cysteine amino terminal). This peptide is predicted to be cytoplasmic (2).
Purity:
Serum.
Form
Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes rat Aquaporin 4 (AQ, WCH4; MIWC). Immunizing sequence is unique to AQP4. Species Sequence Homology: mouse: 94%; bovine 88%: rabbit: 86%: human: 82%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Leung JCK, 2005, Nephrology 10, 63-72, , WB,, IHC 2. Bobic M, 2004, Brain Research, 1026, 168-178, , IHC, IF, EM 3. Cadnapaphornchai MA, 2004, AJP Renal Physiol, 286, 875, WB, 4. Blank M, 2004, Mol Cellular Neuroscience, 26, 530-543, , IF 5. Meng S, 2004, Eur J Neuroscience 19, 2261-2269, IHC 6. Lim S-W, 2004, AJP Renal Physiol,; 287: 139 - 151, WB, IHC 7. Amiry-Moghaddam M, 2003, PNAS,; 100: 2106 - 2111., , IHC 8. Speake T, 2003, BBA, 1609, 80-86, WB, IF 9. Tietz PS, 2003, JBC 278, 20413-20419, WB, 10. Saadoun, S., et al., Neurology, Neurosurgery and Psychiatry 72: 262-265 (2002). 11. Hasegaw,a et al., J. Biol. Chem. 269: 5497–5500 (1994). 12. Jung,, J.S., et al., PNAS 91: 13052-13056 (1994). 13. Terris, J., et al., Am. J. Physiol. 269: F775-85 (1995). 14. Fregeri, A., et al., PNAS 92: 4328-4331 (1995). 15. Misaka, T., et al., FEBS Letters 381: 208-212 (1996).