Technical Data
A3000-20
Aquaporin 5 (AQP5)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin”. Over the last decade, genes for several members of aquaporin family (AQP1-9 and AQPAP) have been cloned, expressed, and their distribution studied in many tissues. AQP5 (human, mouse, and rat 265aa) is found in several epithelial tissues (salivary and lachrymal glands, corneal epithelium in the eye and lung). It is implicated in generation of saliva, tears, and pulmonary secretions. AQP families of proteins are predicted to contain six transmembrane domains.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5-1ug/ml; Coat plates with control peptide at 1ug/ml.
Western Blot: 1-10ug/ml (ECL)
Immunohistochemistry (Paraformaldehyde fixed tissues): 2-10ug/ml
Immunofluorescence (Paraformaldehyde fixed tissues): 2-10ug/ml
Note: The use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended to suppress non-specific bands.
Optimal dilutions to be determined by the researcher.

Control Peptide: A3000-23: Aquaporin 5 (AQP5), Rat

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgG1Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRatRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Synthetic peptide corresponding to 17aa of rat Aquaporin 5 conjugated to KLH. Epitope: C-terminal. Species Sequence Homology: mouse, 100%; human, 80%; sheep, 76%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:
Reconizes rat Aquaporin 5 (AQP5). No significant homology is seen with other AQPs or any other protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Borok, Z., AJP Lung Cell Mol. Physiol. 282: L599 (2002). (IF) 2. Blank, M., Mol. Cell. Neurosci. 26: 530-543 (2004). (IF) 3. Chen, J., Lab. Invest. 84: 727-735 (2004). 4. Li, J., Lab. Invest. 84: 1430-1438 (2004). (IHC) 5. Borok, Z., Am. J. Respir. Cell Mol. Bio. 18: 554-561 (1998). 6. Beroukas, D., Lancet 358: 1875-1876 (2001). (IHC) 7. Larina, O., J. Cell Sci. 118: 4131-4139 (2005). (IF) 8. Lindsay, L.A., Acta Histochemica 106: 299-307 (2004). (IF) 9. Rabb, H., Kidney Intl. 63(2): 600-606 (2003). 10. Wang, W., Calcified T Intl. 72(3): 222-227 (2003). 11. Raina, S., et al., J. Biol. Chem. 270: 1908-1912 (1995). 12. Lee, M.D., et al., J. Biol. Chem. 271: 8599-8604 (1996). 13. Krane, C.M., et al., Mamm. Genome 10: 498-505 (1999).