Technical Data
A3000-25A
Aquaporin 6 (WCH3, hKID, AQP2-L)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane.

AQP6 (WCH3 or hKID or AQP2-like; 282 aa; 29kD; chromosome 12q13) is found only in the kidney with low water permeability. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. AQP6 shows greatest homology with hMIP (48%) and hAQP-2 (52%). It also has similarity with human MIWC (AQP4; 34%), CHIP-28 (AQP1; 38%), and GLIP (AQP3; 22%).

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Immunohistochemistry & Immunofluorescence: we recommend the use of affinity purified antibody at 2-10ug/ml in paraformaldehyde fixed sections of tissues. Neat serum can be used at 1:500 or more.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgYSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanChicken
Concentration:
As reported
Immunogen:
A 19 AA synthetic peptide within the carboxy terminal domain of human AQP6 (1). This domain is predicted to be cytoplasmic.
Purity:
Serum
Form
Supplied as a liquid, neat serum, 40% glycerol.
Specificity:
The 19 AA peptide was found unique to AQP6 without significant homology to any other AQPs. AQP6 from other species are yet to be cloned. Antibody crossreactivity in various species is not known.Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Ma T et al (1996) Genomics 35, 543-550.