Technical Data
A3000-26
Aquaporin 6 (WCH3, hKID, AQP2-L)
Description:
AQP6 (WCH3 or hKID or AQP2-like; 282 aa; 29kD; chromosome 12q13) is found only in the kidney with low water permeability. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. AQP6 shows greatest homology with hMIP (48%) and hAQP-2 (52%). It also has similarity with human MIWC (AQP4; 34%), CHIP-28 (AQP1; 38%), and GLIP (AQP3; 22%).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution
Western Blot: 1:1000-1:5000 using Chemiluminescence technique.
ELISA: 1:10,000-1:50,000. Coat ELISA plate with 1ug/ml control peptide.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
A3000-25D: Aquaporin 6, Human, Control Peptide (WCH3, hKID, AQP2-L)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide, 19aa corresponding within the carboxy terminal domain of human AQP6 (KLH). Cellular Localization: Cytoplasmic. Species Sequence Homology: rat: 47%.
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes human Aquaporin 6. Epitope recognized by this antibody is not found in human AQP6 isoform 2. No significant homology to AQP2 or any other AQPs.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ma T et al (1996) Genomics 35, 543-550. 2. Jeremic A, 2005, Exp. Biol. Med 230: 674-680. 3. WAng, W, 2003 Calcified T. Intl 72(3): 222-227