Technical Data
A3000-30
Aquaporin 7 (rAQP7)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. AQP7 (rAQP7, 269aa) is abundantly expressed in testis. It is involved in water, glycerol and urea transport. AQP7 is also found in adipose tissue, kidney, and heart. AQP6 has highest homology (48%) with AQP3. AQP7 has been localized to in the spermatids and at maturing sperms. AQP families of proteins are predicted to contain six transmembrane domains.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5-1ug/ml using controlpeptide (Cat #A3000-31F) to coat plates to 1ug/ml
Western Blot: 1-10ug/ml (ECL)
Optimal dilutions to be determinedby the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
A sysnthetic peptide corresponding to 18aa near the N-terminal, cytoplasmic domain of rat Aquaporin 7, conjugated to KLH.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes rat Aquaporin 7. No significant homology with human AQP7, AQP7-like or AQP7-adipose. Species Sequence Homology: mouse, 81%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ishibashi K et al (997) JBC 272, 20782