Technical Data
A3000-35
Aquaporin 8 (AQP8)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin”. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein.

Applications:
Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml
ELISA: 0.5-1ug/ml
Immunohistochemistry and Immunofluorescence: 2-10ug/ml in a paraformaldehyde fixed sections of tissue.
Optimal dilution to be determined by researcher

NOTE: We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
A 16 AA synthetic peptide within the C-terminal, cytoplasmic domain of rat Aquaporin-8 (Accession #P56405). Species sequence homology: 93% mouse, 81% human.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul of PBS.
Specificity:
Recognizes rat Aquaporin 8.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Koyama, Yu et al (1997) JBC 272:30329; 2. Ishibashi K et al (1997) BBRC 714:714-718. Citation: Elkjær M-L, 2002, Am J Physiol Renal Physiol 281: F1047, WB/IHC Barcroft LC, 2003, Developmental Biol. 256, 342-354. WB, IHC Ueno Y 2004, Liver Int. 23, 449-459, WB, IFElkjær M-L, 2002, Am J Physiol Renal Physiol 281: F1047, WB/IHC Barcroft LC, 2003, Developmental Biol. 256, 342-354. WB, IHC Ueno Y 2004, Liver Int. 23, 449-459, WB, IF
1. Koyama, Yu et al (1997) JBC 272:30329; Ishibashi K et al (1997) BBRC 714:714-718.3. Elkjær M-L, 2002, Am J Physiol Renal Physiol 281: F1047. 4. Barcroft LC, 2003, Developmental Biol. 256, 342-354.