Technical Data
A3000-36A
Aquaporin 8
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin”.
A new water channel, AQP8, has been identified in rat pancreas and testis by homology cloning. AQP8 (263aa; 28kD) is also found in liver, colon and salivary glands. AQP8 is expressed in all stages of spermatogenesis. Unlike other AQP, AQP8 has unusually long N-terminus and a short C-terminus. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. AQP8 has significant homology to various AQPs: g-TIP (plant water channel, 37%); AQP2 and MIP (37%), AQP1, AQP4, and AQP5 (30-34%); AQP3 (26%). AQP8 does not facilitate glycerol transport.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000 using ECL.
ELISA: 1:10,000-50,000. Control peptide can be used to coat plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Control Peptide:
A3000-36F: Aquaporin 8, Rat, Control Peptide (AQP8)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20°CBlue IceRatRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to 16aa near the C-terminal , cytoplasmic domain of rat AQP8. Species Sequence Homology: mouse: 93%; human: 81%
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide
Specificity:
Recognizes rat AQP8.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Koyama, Y., et al., JBC 272: 30,329 (1997). 2. Ishibashi, K., et al., BBRC 714: 714-718 (1997).