Aquaporin 8, Rat, Control Peptide (AQP8)
|Molecular Biology||Storage: -20°CShipping: Blue Ice|
A 16aa synthetic peptide within the C-terminal domain of rat AQP8.
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane.
A new water channel, AQP8, has been identified in rat pancreas and testis by homology cloning (1). AQP8 (263 aa; 28kD) is also found in liver, colon and salivary glands. AQP8 is expressed in all stages of spermatogenesis. Unlike other AQP, AQP8 has unusually long N-terminus and a short C-terminus. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. AQP8 has significant homology to various AQPs: g-TIP (plant water channel, 37%); AQP2 and MIP (37%), AQP1, AQP4, and AQP5 (30-34%); AQP3 (26%). AQP8 does not facilitate glycerol transport.
Control peptide for A300-36A (antiserum) and A3000-36 (affinity purified).
Supplied as a liquid in PBS, pH 7.5, 0.1% sodium azide.
16aa synthetic peptide within the C-terminal domain of rat AQP8.
Unique to AQP8 without significant homology to any other AQPs.
Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.
Antibody Blocking: Use 5-10ug per 1ug of affinity purified antibody (A3000-36) or 1ul of antiserum (A3000-36A).
ELISA: 50-100ng control peptide per well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity: Highly purified
Form: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide
Specificity: Unique to AQP8 without significant homology to any other AQPs. It has 93% homology with the mouse AQP8. AQP7 from other species are yet to be cloned. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
(1) Koyama, Yu et al (1997) JBC 272, 30329; Ishibashi K et al (1997) BBRC 714, 714-718.