Technical Data
A3000-75
Aquaporin 10 (AQP10)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. A new member of AQP family, AQP10, has been cloned (1). AQP10 (human 264aa) is most closely related with AQP3 (53%), AQP9 (52%), and AQP7 (43%). It is abundantly expressed in duodenum and jejunum. AQP10 is not permeable to urea and glycerol.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:50,000; Control peptide (A3000-70) can be used to coat ELISA plates at 1ug/ml.
Western Blot: 1:1000-1:5000

Note: We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.

Optimal dilutions to be determined by the researcher.

Control Peptide: A3000-70 Aquaporin 10, Human, Control Peptide (AQP10)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A 17aa synthetic peptide within the cytoplasmic, C-terminal region of human AQP10 coupled to KLH
Purity:
Serum
Form
Supplied as a liquid in PBS, pH 7.4, 40% glycerol.
Specificity:
Recognizes human Aquaporin 10. The 17aa peptide is unique to AQP10 without significant homology to any other AQPs.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hatakeyama, S., et al., BBRC 287: 814-819 (2001).