		Technical Data
A3000-90B	Aquaporin 12 (AQP12, Aquaporin-12A, AQP-12, Aquaporin-12, AQPX2)

Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending
thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin.”

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ug/ml. Control peptide, A3000-90D, can be used to coat plates at 1ug/ml.
Western Blot: 1-10ug/ml using ECL. In order to suppress non-specific bands the use of 0.5%-1% milk is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to 18aa from the C-terminus of human Aquaporin 12 (Accession #Q8IXF9) (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.

Specificity:

Recognizes human Aquaporin 12.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Itoh T, et al (2005) BBRC 330(4), 832-838.