Technical Data
A3002-25
Aquaporin, Adipose Specific (AQPAP, AQP7-Like)
Description:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane. Most recently, adipose specific AQP-adipose (342 aa; AQPAP or AQP7-like) has been cloned. It facilities water and glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot :1:1000-1:5000 using ECL.
ELISA: 1:100,000. A3002-35 Control peptide can be used to coat ELISA plates at 50-100ng/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanChicken
Concentration:
Not determined
Immunogen:
20 AA synthetic peptide within the N-terminal domain of human AQPAP (1). Predicted to be cytoplasmic.
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes human Aquaporin, Adipose Specific. Species sequence homology: chimp and monkey 100%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kuriyama H et al (1997) BBRC 241, 53-58.