Technical Data
A3305-37
Arachis hypogaea (PNA, Peanut Agglutinin) (FITC)
Description:
Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Arachis hypogaea (PNA, Peanut Agglutinin), 110kD, is composed of four identical subunits of approximately 27kD each, is galactose-specific, with strong anti-T-antigen activity. T-antigen") is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase-treated red blood cells.The presence of Ca++ can enhance the binding of PNA to receptors. Asialo GM1 ganglioside is also thought to be a major cell surface receptor for PNA. PNA is useful in normal and tumor tissue determination for transitional mucosa malignancies.

Applications:
Suitable for use in Immunodiffusion, Immunohistochemistry, Immunocytochemistry and IEP. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 20-100ug/ml
Immunocytochemistry: 100ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
1ml-20CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Arachis hypogaea (PNA, peanut)
Purity:
Purified by ammonium sulfate precipitation
Form
Supplied as a liquid in PBS, pH 7.23 0.05% sodium azide. Labeled with FITC. OD 495/280: 1.53
Specificity:
Recognizes Arachis hypogaea (PNA, peanut).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Immbar M., et al., 1973, IInt. J. of Cancer, 12:93-99. 2. Phiss K., 1977, Experimental Pathology, 14:S15.