Technical Data
A3305-39
Arachis hypogaea (PNA, Peanut Agglutinin) (Texas Red)
Description:
Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.
Arachis hypogaea (PNA, Peanut Agglutinin), 110kD, is composed of four identical subunits of approximately 27kD each, is galactose-specific, with strong anti-T-antigen activity. T-antigen") is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase-treated red blood cells. The presence of Ca++ can enhance the binding of PNA to receptors. Asialo GM1 ganglioside is also thought to be a major cell surface receptor for PNA. PNA is useful in normal and tumor tissue determination for transitional mucosa malignancies.

Applications:
Suitable for use in Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Carbohydrate Specificity: Terminal b-Galactose

Inhibitory Carbohydrate: Lactose > b-Galactose

Activity: 1ug/ml will agglutinate human erythrocytes neuraminidase treatment of the cells.

Texas Red /Protein Ratio (OD595/OD280): 2.4

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Texas Red® conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml-20°CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Arachis hypogaea (PNA, peanut).
Purity:
Purified by gel filtration.
Form
Supplied as a liquid in 0.02M sodium bicarbonate, pH 9.0-9.5. Labeled with Texas red.
Specificity:
Recognizes Arachis hypogaea (PNA, peanut).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Immbar, M. et al., Intnl. J. of Cancer, 12: 93-99 (1973) 2. Phiss, K., Exp. Pathol. 14: 515 (1977). 3. Newman R.A. et al., Hoppe-Seyler’s Z. Physiol. Chem. 358:1517 (1977) 4. Novogrodsky et al., J. Immunol. 115:1243 (1975)