Technical Data
A3309-01C
ARAP1 (Arf-GAP with Rho-GAP Domain, ANK Repeat and PH Domain-containing Protein 1, Centaurin-delta-2, Cnt-d2, CENTD2, KIAA0782)
Description:
ARAP1 is a 1450aa containing signaling protein, belonging to the centaurin family with SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, ARAP1 displays RHO-GAP and phosphatidylinositol (3, 4, 5) trisphosphate (PIP3)-dependent ARF-GAP activity that regulates ARF-, RHO-, and CDC42-dependent cell activities. It associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thus involved in both signal transduction and cellular trafficking transport of a receptor protein involved in apoptosis. This protein is likely involved in the regulation of the cell-specific trafficking of TNFRSF10A and might thus affect the efficacy of TRAIL-induced apoptosis. ARAP1, an adaptor protein, is ubiquitously expressed in most of the tissues.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: >1:32,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to AFTADPLSLLRNV, from the C-terminus of the protein sequence according to NP_056057; NP_001035207
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes ARAP1. Species sequence homology: mouse and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Miura K, Jacques KM, Stauffer S, Kubosaki A, Zhu K, Hirsch DS, Resau J, Zheng Y, Randazzo PA. ARAP1: a point of convergence for Arf and Rho signaling. Mol Cell. 2002 Jan;9(1):109-19. PMID: 11804590