Technical Data
A3309-10B
ARAP3 (Arf-GAP with Rho-GAP Domain, ANK Repeat and PH Domain-containing Protein 3, Centaurin-delta-3, Cnt-d3, CENTD3)
Description:
ARAP3, also known as Centaurin-delta-3, is a 1544aa containing protein with an Arf-GAP domain, three PH domains, a Ras-associating domain, a Rho-GAP domain and a SAM (sterile alpha motif) domain. ARAP3 is a phosphatidylinositol-3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. It is activated by phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding and can be activated by phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. ARAP3 acts on ARF6, RAC1, RHOA and CDC42 and also plays a role in the internalization of anthrax toxin.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to aa323-338 DKDPFPKGVIPLTAIE of human ARAP3.
Purity:
Serum.
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity:
Recognizes human ARAP3. Species Crossreactivity: chimpanzee, canine, mouse, rat, rhesus monkey. Species sequence homology: drosophila, opossum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Krugmann, S. et al. Mol Cell. 9:95-108 (2002).