Technical Data
A3309-10E
ARAP3 (Arf-GAP with Rho-GAP Domain, ANK Repeat and PH Domain-containing Protein 3, Centaurin-delta-3, Cnt-d3, CENTD3)
Description:
ARAP3, also known as Centaurin-delta-3, is a protein with an Arf-GAP domain, three PH domains, a Ras-associating domain, a Rho-GAP domain and a SAM (sterile alpha motif) domain. ARAP3 is a phosphatidylinositol-3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. It is activated by phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding and can be activated by phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. ARAP3 acts on ARF6, RAC1, RHOA and CDC42 and also plays a role in the internalization of anthrax toxin.

Applications:
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:4000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to CTSSPPSSQPLT, from CENTD3, at C-terminal (NP_071926.4).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes CENTD3. Species sequence homology: Bovine, canine, human, mouse and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yagi R, Tanaka M, Sasaki K, Kamata R, Nakanishi Y, Kanai Y, Sakai R. ARAP3 inhibits peritoneal dissemination of scirrhous gastric carcinoma cells by regulating cell adhesion and invasion. Oncogene. 2010 Nov 15.