Technical Data
A3327
Arc p34 (ARP2/3 Complex 34kD subunit, Actin-related Protein 2/3 Complex subunit 2)
Description:
The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml detects p34-ARC in RIPA lysates from human A431, Jurkat and mouse 3T3 cells. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p34-Arc (1ug/ml). Proteins were visualized using a goat anti-rabbit IgG labeled with HRP* and a chemiluminescence detection system.
Immunohistochemistry: 10ug/ml detects p34-Arc in mouse array tissue and formalin-fixed, paraffin-embedded human adenocarcinoma tissue.
Immunocytochemistry: Detects p34-Arc/ARPC2 in C2C12 cells.
Optimal dilution determined by the researcher.

*Recommended Secondary Antibodies
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Included Positive Antigen Control:
A0001-50: A431 Cell Lysate, Non-Stimulated. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to C-EKKEMKTITGKTFS, aa285-298 from human Arc p34 containing a N-terminal cysteine conjugated to KLH.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity:
Recognizes human ARC p34 at ~34kD of the Arp2/3 complex. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Millard, TH., et al., Cell Motil. Cytoskel. 54: 81-90, 2003.