Technical Data
A3330-02
ARF, p14
Description:
The INK4a-ARF locus encodes two unrelated proteins both of which function in tumor suppression. p14ARF arrests the cell cycle in a p53-dependent manner. p14ARF binds to mdm2 and promotes the rapid degradation of mdm2 protein. This interaction is mediated by the exon 1b-encoded N-terminal domain of p14ARF and a C-terminal region of mdm2. ARF-mediated mdm2 degradation is associated with mdm2 modification and concurrent p53 stabilization and accumulation. Deletion of the ARF-INK4a locus simultaneously impairs both the INK4a-cyclin D/cdk4-RB and the ARF-mdm2-p53 pathways.

Positive Control: HeLa cells.

Applications and Suggested Dilutions:
Immunofluorescence
Immunoprecipitation (Use Protein G)
(Ab 2mg/mg protein lysate)
Western Blotting (Ab 2-4mg/ml for 2hrs at RT)

Cellular Localization: Nuclear
TypeIsotypeCloneGrade
MabIgG10.N.479Affinity Purified
SizeStorageShippingSourceHost
250ul-20°CBlue IceHumanMouse
Concentration:
0.2mg/ml
Immunogen:
Recombinant p14ARF protein.
Purity:
Purified by Protein G affinity chromatography.
Form
10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide.
Specificity:
Recognizes human p14ARF. Does not react with mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.