		Technical Data
A3330-02C	ARF, p14 (p14ARF )

Description:
Applications:
Suitable for use in Immunofluorescence, ELISA Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	8J304	Affinity Purified

Size		Storage	Shipping	Source	Host
250ug		-20°C	Blue Ice		Mouse

Concentration:

~1mg/ml

Immunogen:

His-tagged p14ARF protein expressed in bacteria.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

Specificity:

Species Crossreactivity: Crossreacts with Human. Not yet tested in other species.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

This product has been used in:Clark PA et al. Multiple interacting domains contribute to p14ARF mediated inhibition of MDM2. Oncogene 21:4498-507 (2002). Llanos S et al. Stabilization of p53 by p14ARF without relocation of MDM2 to the nucleolus. Nat Cell Biol 3:445-52 (2001).