Suitable for use in Immunofluorescence, ELISA Western Blot and Immunoprecipitation. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|His-tagged p14ARF protein expressed in bacteria.|
|Purified by Protein A affinity chromatography.|
|Supplied as a liquid in PBS, pH 7.2. No preservative added.|
|Species Crossreactivity: Crossreacts with Human. Not yet tested in other species.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
This product has been used in:Clark PA et al. Multiple interacting domains contribute to p14ARF mediated inhibition of MDM2. Oncogene 21:4498-507 (2002). <12085228>Llanos S et al. Stabilization of p53 by p14ARF without relocation of MDM2 to the nucleolus. Nat Cell Biol 3:445-52 (2001). <11331871>|