Technical Data
A3330-11A
ADP-Ribosylation Factor Domain Protein 1 (ARD1, ARFD1, ARF domain protein 1, GTP-binding Protein ARD-1, Tripartite Motif Protein 23, TRIM23, E3 Ubiquitin-protein Ligase TRIM23, RING Finger Protein 46, RNF46)
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Technical Data |
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A3330-11A |
ADP-Ribosylation Factor Domain Protein 1 (ARD1, ARFD1, ARF domain protein 1, GTP-binding Protein ARD-1, Tripartite Motif Protein 23, TRIM23, E3 Ubiquitin-protein Ligase TRIM23, RING Finger Protein 46, RNF46) |
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Description: The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARFBP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents. Applications: Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Immunocytochemistry: 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Daudi cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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