		Technical Data
A3330-12A	ARFBP1, CT (HUWE1, E3 Ubiquitin-protein Ligase HUWE1, ARF-binding Protein 1, ARF-BP1, HECT, UBA and WWE Domain-containing Protein 1, Homologous to E6AP Carboxyl Terminus Homologous Protein 9, HectH9, Large Structure of UREB1, LASU1, Mcl-1 Ubiquitin Ligase

Description:
HUWE1 is a member of the HECT E3 ubiquitin ligase family. The HECT domain lies in the C-terminus and contains the active-site cysteine which forms an intermediate ubiquitin-thioester bond. E3 family members are divided into three subfamilies based on their protein-protein interaction domains; this gene encodes a member of the SI(ngle)-HECT E3 subfamily. In lung, breast, and colorectal carcinomas, this gene is highly expressed. Mutations in this gene has been found in 3 unrelated families with X-linked syndromic mental retardation, Turner type.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to 19aa from human ARF-BP1 at C-terminal (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

Specificity:

Recognizes human ARF-BP1. Species sequence homology: Mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.