		Technical Data
A3334-95	ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 1 (ASAP1, AMAP1, Centaurin beta 4, CENTB4, Development and Differentiation Enhancing Factor 1, DDEF1, KIAA1249, PAG2, PAP, ZG14P) (Azide Free)

Description:
Applications:
Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
ELISA: 1ug/ml-3ng/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	10C3	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to a portion of amino acids within aa1030-1130 of human DDEF1.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).

Specificity:

Recognizes human DDEF1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.