Technical Data
A3334-96
ASAP2 (Arf-GAP with SH3 Domain, ANK Repeat and PH Domain-containing Protein 2, Development and Differentiation-enhancing Factor 2, Paxillin-associated Protein with ARF GAP Activity 3, PAG3, Pyk2 C-terminus-associated Protein, PAP, DDEF2, KIAA0400)
Description:
ASAP2 is a multidomain protein belonging to the beta family with two ANK repeats, an Arf-GAP domain, a PH domain and a SH3 domain. This protein localizes in the Golgi apparatus and also at the plasma membrane where it colocalizes with protein tyrosine kinase 2-beta (PYK2) and forms a stable complex with PYK2 in vivo. ASAP2 is tyrosine phosphorylated by activated PYK2. It functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. ASAP2 activates the small GTPases ARF1, ARF5 and ARF6 and regulates the formation of post-Golgi vesicles and modulates constitutive secretion. It modulates phagocytosis mediated by Fc gamma receptor and ARF6. Usually detected in heart, brain, placenta, kidney, monocytes and pancreas, it is up-regulated during monocyte maturation. Multiple transcript variants encoding different isoforms have been found.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Brain lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa700-750 of human ASAP2.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human ASAP2. Species Crossreactivity: chimpanzee, mouse, opossum, rat, rhesus monkey. Species sequence homology: chicken, bovine, canine, equine, xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ishikawa, K. et al. DNA Res. 4:307-313 (1997). 2. Andreev, J. et al. Molec. Cell. Biol. 19:2338-2350 (1999).