Technical Data
L-Arginine, Conjugated
Applications: Suitable for use in ELISA. Other applications not tested.

Using a conjugate Arginine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :

Compound Cross-reactivity ratio (a)
Arginine-G-BSA 1
Glutamine-G-BSA 1/> 100,000
Asparagine-G-BSA 1/> 100,000
Lysine-G-BSA 1/> 100,000

(a): Arginine-G-BSA concentration/unconjugated or conjugated amino acids concentration at half displacement; G = Glutaraldehyde, BSA: Bovine Serum Albumin.

Recommended dilution
The anti-conjugated Arginine antibodies can be diluted between 1/2,000-1/10,000.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20CBlue IceRabbit
Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Recognizes conjugated L-Arginine. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
KING M.G., GEFFARD M., CHAUVEAU J., GAFFORI O., LE MOAL M. and MUYARD J.P. Des tyr-g-endorphin (DTgE) and pineal levels of melatonin and arginine vasopressin (AVP). Eur. J. Pharmacol., (1981) 76, 271-274. KING M.G., LE MOAL M., STINUS L. and GEFFARD M. Central administration of arginine-vasotocin: effect of exploratory behavior in the rat. Psychopharmacology, (1982) 76, 40-43.