Technical Data
Arginine, symmetric, dimethyl (SYM10)
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: A 1:500-1:1000 detects methylated p60 (unidentified protein) and Sm protein B, B and D in RIPA lysates from Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to PVDF and probed with A3550-07C (1:1000). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Detects bands at 60, 30, 32 and 16kD, corresponding to p60, Sm B, B and D, respectively.
Immunoprecipitation: Reported by an independent laboratory to immunoprecipitate proteins containing symmetric dimethyl-arginine.
Immunocytochemistry: 1:500. Reported by an independent laboratory to immunostain Cajal bodies in the nuclei of HeLa, WI-38 and NIH/3T3 cells fixed with paraformaldehyde and permeabilized with triton.
Optimal dilutions to be determined by the researcher.

Included Positive Antigen Control:
J9000: Jurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
200ul-20CBlue IceRabbit
Not Determined
Synthetic peptide corresponding to four symmetrical dimethyl-arginine-glycine repeats (KR*GR*GR*GR*G in which (R*) denotes symmetric dimethylation of arginine). Conjugated to KLH.
Supplied as a liquid, 0.05% sodium azide, 30% glycerol.
Recognizes proteins that contain multiple symmetrically dimethylated arginines, including the Sm protein B, B and D. Does not recognize non-methylated peptides, peptides containing asymmetrically dimethylated arginines, or a peptide containing a single symmetric dimethyl-arginine. Species Crossreactivity: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Boisvert, F.M., et al., J. Cell. Biol. 159: 957-969, 2002. 2. Boisvert, F.M., et al., Mol. Cell. Proteomics 2: 1319-30, 2003.