		Technical Data
A3570-21	Argonaute 1, 2, 3, 4 (Eukaryotic Translation Initiation Factor 2C 1, 2, 3, 4)

Description:
Small interfering RNAs (siRNAs) have gained much attention for their powerful ability to suppress gene expression. Introduction of double-stranded RNA (dsRNA), that are homologues in sequence to a gene, has proven to suppress that gene's expression through a process known as RNA interference (RNAi); this process post-transcriptionally silences a gene through mRNA inhibition or degradation. Both biochemical and genetic studies have led to the identification of two, conserved gene families that are universal components of the interference process. These are the Dicer family, which is comprised of members of the Ribonuclease III family of enzymes, and the Argonaute gene family. Argonaute proteins addition to their role in RNAi process, may also influence development, such as, stem cell fate determination.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined.

Immunogen:

Synthetic peptide corresponding to aa361-378 (TSTMIRATARSAPDRQEE) of human Argonaut 2. (Genbank accession number: NP_036286).

Purity:

Serum.

Form

Supplied as a liquid, 0.025% sodium azide, 50% glycerol.

Specificity:

Recognizes human Argonaut 2, Argonaut 3 and Argonaut 4. Sequence Homology: Argonaut 1 (94%). Species sequence homology: mouse, rat, bovine, chicken.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Carmell,M.A., Xuan,Z., Zhang,M.Q. and Hannon,G.J. The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis. Genes Dev. 16 (21), 2733-2742 (2002). 2. Doi,N., Zenno,S., Ueda,R., Ohki-Hamazaki,H., Ui-Tei,K. and Saigo,K. Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and IF2C translation initiation factors. Curr. Biol. 13 (1), 41-46 (2003).