		Technical Data
A3570-21C	Argonaute 2, 3, 4 (Eukaryotic Translation Initiation Factor 2C 2, 3, 4)

Description:
Argonaut 2,3,4, also known as Eukaryotic translation initiation factor 2C, is a member of the Argonaut family of proteins with 861aa. It is a highly basic protein with PAZ and PIWI domains and is well known for its role in short-interfering-RNA-mediated gene silencing (TGS pathway) and RNA interference. It shows identity with rabbit translation initiation factor Eif2c and is necessary for maintaining non-CpG DNA and histone methylation, developmental control, stem cell maintenance, and tumorigenesis. Reports suggest that Argonaut 2,3,4 may be a component of a hypothetical meristem forming competence factor. RT-PCR and ELISA revealed intermediate and nearly uniform levels of expression in all tissues.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined.

Immunogen:

Synthetic peptide corresponding to aa132-148 HLKSFTEQLRKISRDAG of human Argonaut 2,3,4.

Purity:

Serum

Form

Supplied as a liquid, 0.025% sodium azide, 50% glycerol.

Specificity:

Recognizes human Argonaut 2,3,4. Species Crossreactivity: chimpanzee, bovine, canine, equine, mouse, opossum. Species sequence homology: pufferfish, zebrafish.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Carmell, MA. et al. Genes Dev. 16:2733-2742 (2002). 2. Daniel, Zilberman. et al. Science. 299:716-719 (2003). 3. Meister, G. et al. Molecular Cell. 15:185-197 (2004). 4. Nagase, T. et al. DNA Res. 7:273-281 (2000).