Technical Data
Argonaute 2, 3, 4 (Eukaryotic Translation Initiation Factor 2C 2, 3, 4)
Argonaut 2,3,4, also known as Eukaryotic translation initiation factor 2C, is a member of the Argonaut family of proteins with 861aa. It is a highly basic protein with PAZ and PIWI domains and is well known for its role in short-interfering-RNA-mediated gene silencing (TGS pathway) and RNA interference. It shows identity with rabbit translation initiation factor Eif2c and is necessary for maintaining non-CpG DNA and histone methylation, developmental control, stem cell maintenance, and tumorigenesis. Reports suggest that Argonaut 2,3,4 may be a component of a hypothetical meristem forming competence factor. RT-PCR and ELISA revealed intermediate and nearly uniform levels of expression in all tissues.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined.
Synthetic peptide corresponding to aa132-148 HLKSFTEQLRKISRDAG of human Argonaut 2,3,4.
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Recognizes human Argonaut 2,3,4. Species Crossreactivity: chimpanzee, bovine, canine, equine, mouse, opossum. Species sequence homology: pufferfish, zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Carmell, MA. et al. Genes Dev. 16:2733-2742 (2002). 2. Daniel, Zilberman. et al. Science. 299:716-719 (2003). 3. Meister, G. et al. Molecular Cell. 15:185-197 (2004). 4. Nagase, T. et al. DNA Res. 7:273-281 (2000).