Technical Data
Argonaute 4 (Eukaryotic Translation Initiation Factor 2C 4)
Argonaute 4, a member of the Argonaute family of proteins, is a highly basic protein containing PAZ and PIWI domains. These proteins are well known for their role in short-interfering-RNA-mediated gene silencing (TGS pathway) and RNA interference. Reports suggest that Argonaute 4 may be a component of a hypothetical meristem forming competence factor. RT-PCR and ELISA revealed intermediate and nearly uniform levels of expression in all tissues. They show identity with rabbit translation initiation factor Eif2c and are also necessary for maintaining non-CpG DNA and histone methylation, developmental control, stem cell maintenance and tumorigenesis.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined.
Synthetic peptide corresponding to aa627-640(CRQEISQELLYSQEV) of human Argonaut 4.
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Recognizes human Argonaut 4. Species Crossreactivity: chimpanzee, bovine, canine, equine, monkey. Species sequence homology: chicken, mouse, rat, xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Carmell, MA. et al. Genes. Dev. 16:2733-2742 (2002). 2. Meister, G. et al. Molecular. Cell. 15:185-197 (2004). 3. Nagase, T. et al. DNA. Res. 7:273-281 (2000).