Technical Data
A3570-25
Arginyl-tRNA Synthetase (RARS, MGC8641, DALRD1, ArgRS, arginyl-tRNA synthetase, arginine tRNA ligase 1, cytoplasmic)
Description:
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:128,000
Western Blot: 1-3ug/ml ~75kD band observed in lysates of HeLa and HepG2 (calculated MW of 75.4kD according to NP_002878.2).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-SLQAERNKPTKN, from the internal region of human Arginyl-tRNA Synthetase (NP_002878.2).
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human Arginyl-tRNA Synthetase. Species sequence homology: mouse, rat, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bottoni A, Vignali C, Piccin D, Tagliati F, Luchin A, Zatelli MC, Uberti EC, Proteasomes and RARS modulate AIMP1/EMAP II secretion in human cancer cell lines. Journal of cellular physiology 2007 Aug 212 (2): 293-7.