Technical Data
ARHGEF4 (APC-stimulated Guanine Nucleotide Exchange Factor, Asef, GEF4, GEF-4, KIAA1112, Rho Guanine Nucleotide Exchange Factor (GEF) 4, Rho Guanine Nucleotide Exchange Factor 4, STM6)
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through Gucleotide exchange protein coupled receptors. Acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. This protein is similar to rat collybistin protein. Alternative splicing of the gene generates two transcript variants which encode different isoforms.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000.
Western Blot: 2-4ug/ml. ~ 80kD band observed in Human Brain lysates (calculated MW of 79.1kD according to NP_056135 and 76.4kD according to 127462).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide PWEEPAGEKPSCS, representing the N-Terminus of the human protein (residues 2-13), according to NP_056135; NP_127462.
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide.
Species Crossreactivity: Reacts with Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.