Technical Data
A3570-69
ARHGAP20 (Rho GTPase-activating Protein 20, Rho-type GTPase-activating Protein 20, KIAA1391)
Description:
ARHGAP20 is a potent GTPase-activating protein (GAP) and convert Rho-type GTPases into an inactive GDP-bound state. It contains a pleckstrin homology (PH) domain, a Ras-associating domain, a Rho-GAP domain and two Annexin-like (ANXL) repeats. ARHGAP20 is a direct downstream target of Rap1 in the neurite outgrowth while it's over expression leads to inactivation of Rho for promoting the neurite outgrowth in a Rap1-dependent manner. ARHGAP20 is a tumor suppressor gene and is inactivated by deletion in breast cancer and also by chromosomal translocation in B-cell chronic lymphocytic leukemia. Reports suggest that it may take part in rearrangements of the cytoskeleton and cell signaling events that occur during spermatogenesis. It is expressed predominantly in human brain, liver, ovary and spinal cord while low expression is found in lymph nodes and fetal liver.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 5-8ug/ml
Immunohistochemistry (paraffin): 4-8ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa570-620 of human ARHGAP20.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human ARHGAP20. Species Crossreactivity: chimpanzee. Species sequence homology: bovine, equine, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yamada, T. et al. J. Biol. Chem. 280:33026-33034 (2005). 2. Kalla, C. et al. Genes. Chromosomes. Cancer. 42:128-143 (2005).