Technical Data
A3570-70E
ARIH1 (E3 Ubiquitin-protein Ligase ARIH1, H7-AP2, HHARI, Monocyte Protein 6, MOP-6, Protein Ariadne-1 Homolog, ARI-1, UbcH7-binding Protein, UbcM4-interacting Protein, Ubiquitin-conjugating Enzyme E2-binding Protein 1, ARI, MOP6, UBCH7BP, HUSSY-27)
Description:
E3 ubiquitin-protein ligase, which catalyzes polyubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. May play a role in protein translation by mediating polyubiquitination of EIF4E2, leading to its subsequent degradation.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.2-1ug/ml, U937 lysate observed on ~65kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-HEGYEKDLWEYIED, from human ARIH1, at C-terminal (NP_005735.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human ARIH1. Species sequence homology: Bovine and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. LaVoie MJ, Cortese GP, Ostaszewski BL, Schlossmacher MG. The effects of oxidative stress on parkin and other E3 ligases. J Neurochem. 2007 Dec;103(6):2354-68. 2. Wong ES, Tan JM, Wang C, Zhang Z, Tay SP, Zaiden N, Ko HS, Dawson VL, Dawson TM, Lim KL. Relative sensitivity of parkin and other cysteine-containing enzymes to stress-induced solubility alterations. J Biol Chem. 2007 Apr 20;282(16):12310-8.