Technical Data
A3570-84B
ARL2, NT (ADP-ribosylation Factor-like Protein 2)
Description:
ADP-ribosylation factors (ARFs) regulate intracellular vesicular membrane trafficking and stimulate a phospholipase D isoform. ARL (ADP-ribosylation like factor) proteins are very similar in sequence to ARFs. ARFs and ARF-like (ARL) proteins, which share sequence similarity with ARFs, form a subfamily of the Ras-related GTPase superfamily. ARL2 encodes a ubiquitiously expressed 184aa predicted protein that is 76% identical to ARF1 and 40 to 45% identical to the Drosophila ARL proteins. Recombinant ARL2 binds to GTP rapidly but guanine nucleotide exchange does not require phospholipids, which is a characteristic of the ARF proteins.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the N-terminal region of human ARL2 (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human ARL2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Antoshechkin, I., et al., Dev. Cell 2(5):579-591 (2002). 2. Bhamidipati, A., et al., J. Cell Biol. 149(5):1087-1096 (2000). 3. Clark, J., et al., Proc. Natl. Acad. Sci. USA 90(19):8952-8956 (1993).