Technical Data
Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3 and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin (1). The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation (2). The ARP2/3 complex (along with N-WASP) regulates nuclear actin filament nucleation and controls actin polymerization during transcription (3).

Suitable for use in Western Blot.
Other applications have not been tested.

Recommended Dilutions:
Western blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide (KLH-coupled) corresponding to the sequence of human ARP2.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
Recognizes endogenous levels of total human ARP2 protein. Species crossreactivity: mouse, rat, monkey, hamster and D.melanogaster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Goley, E.D. and Welch, M.D. (2006) Nat. Rev. Mol. Cell Biol. 7, 713-726. 2. Morita, T. et al. (2007) J. Biol. Chem. 282, 8454-8463. 3. Yoo, Y. et al. (2007) J. Biol. Chem. 282, 7616-7623.