Technical Data
A3576
ARPC2 (ARPC2, actin related protein 2/3 complex, subunit 2, 34kDa, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 protein compex subunit 34)
Description:
This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA: >1:32,000.
Western Blot: 0.1-0.5ug/ml. ~33-34kD band observed in Hela and Human Muscle lysates (predicted MW of 34kD according to NP_005722).
Immunohistochemistry: 3-5ug/ml paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Staining (0.2ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See A3576-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-EMKTITGKTFSSR, from the C Terminus of human ARPC2 (NP_005722.1; NP_690601.1).
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes both reported isoforms of human ARPC2. Species Sequence Homology: mouse, rat, canine and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ., The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly., J Cell Biol. 1997 Jul 28;138(2):375-84. 2. Fougner SL, Bollerslev J, Latif F, Hald JK, Lund T, Ramm-Pettersen J, Berg JP.Low levels of raf kinase inhibitory protein in growth hormone-secreting pituitary adenomas correlate with poor response to octreotide treatment.J Clin Endocrinol Metab. 2008 Apr;93(4):1211-6. 3. Sakata D, Taniguchi H, Yasuda S, Adachi-Morishima A, Hamazaki Y, Nakayama R, Miki T, Minato N, Narumiya S.Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1. J Exp Med. 2007 Sep 3;204(9):2031-8.