		Technical Data
A3576-98D	Arrestin 1, beta (ARRB1, ARB1, ARR1)

Description:
The Arrestin family consists of four members: Arrestin 1 (visual Arrestin), Arrestin 2 (beta Arrestin 1), Arrestin 3 (beta Arrestin 2), and Arrestin 4 (cone Arrestin). While visual and cone Arrestins are found almost exclusively in the retina, beta Arrestins 1 and 2 are ubiquitously expressed. Beta Arrestins appear to act as negative regulators on G protein-coupled receptors, (Lefkowitz, 1998). Interaction with molecules such as clathrin, AP-2 and NSF directs the clathrin-mediated internalization of G-protein-coupled receptors. Interaction with molecules such as Src, Raf, Erk, ASK1 and JNK3 appears to regulate several pathways that result in the activation of MAP kinases, (Lin, 1998; Miller, 2001).

Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:500
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HEK293 cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgM,k	9H198	Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to aa357-372 of human beta arrestin 1.

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

Specificity:

Recognizes human beta arrestin 1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Miller, W.E., and R.J. Lefkowitz. (2001). Curr. Opin. Cell Biol. 13: 139-145. 2. Lefkowitz, R.J. (1998). J. Biol. Chem. 273: 18677-80. 3. Lin, F.T., et al. (1998). J Biol Chem. 273: 31640-3.