Technical Data
A3576-98D
Arrestin 1, beta (ARRB1, ARB1, ARR1)
Description:
The Arrestin family consists of four members: Arrestin 1 (visual Arrestin), Arrestin 2 (beta Arrestin 1), Arrestin 3 (beta Arrestin 2), and Arrestin 4 (cone Arrestin). While visual and cone Arrestins are found almost exclusively in the retina, beta Arrestins 1 and 2 are ubiquitously expressed. Beta Arrestins appear to act as negative regulators on G protein-coupled receptors, (Lefkowitz, 1998). Interaction with molecules such as clathrin, AP-2 and NSF directs the clathrin-mediated internalization of G-protein-coupled receptors. Interaction with molecules such as Src, Raf, Erk, ASK1 and JNK3 appears to regulate several pathways that result in the activation of MAP kinases, (Lin, 1998; Miller, 2001).

Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:500
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM,k9H198Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa357-372 from human beta arrestin 1, conjugated to KLH (P49407).
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes human beta arrestin 1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Miller, W.E., and R.J. Lefkowitz. (2001). Curr. Opin. Cell Biol. 13: 139-145. 2. Lefkowitz, R.J. (1998). J. Biol. Chem. 273: 18677-80. 3. Lin, F.T., et al. (1998). J Biol Chem. 273: 31640-3.