Technical Data
A3576A
ARPC2 (Actin Related Protein 2/3 Complex Subunit 2 34kD, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 Protein Compex Subunit 34)
Description:
The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.

Positive Control: Hela and Human Muscle lysates.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000.
Western Blot: 0.1-0.5ug/ml. Detects a band of approximately 34kD in Hela and Human Muscle lysates.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide: EMKTITGKTFSSR, representing the C-Terminus of the human protein (residues 288-300), according to NP_005722; NP_690601.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
Specificity:
This antibody is expected to recognize both reported isoforms as represented by NP_005722 and NP_690601. Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse, Rat and Zebrafish (90% identity with immunogen) due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Welch MD et al. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol 138:375-84 (1997).