		Technical Data
A3577	Arrestin 1, beta, phosphorylated (Ser412) (ARRB1, ARB1, ARR1)

Description:
Beta-arrestin 1 is a member of the arrestin family, which has been implicated in mediating the desensitization and internalization of G protein-coupled receptors (GPCR) (1). Beta-arrestin 1 is constitutively phosphorylated by Erk1/2 at serine 412 of the C-terminus and localized in the cytosol (2). When recruited to the plasma membrane after agonist stimulation of the beta2-adrenergic receptors, beta-arrestin 1 is rapidly dephosphorylated. This dephosphorylation is an essential step of beta-arrestin 1-mediated receptor endocytosis, but it is not required for receptor desensitization (3).

Applications: Suitable for use in ELISA, Western Blotting and Immunoprecipitation. Other applications not tested.

Recommended Dilution: Western Blotting: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:50

Optimal dilutions to be determined by the researcher.

Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	3A14	Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide (KLH coupled) corresponding to residues surrounding Ser412 of human beta-arrestin 1.

Purity:

Purified.

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.

Specificity:

Detects endogenous beta-arrestin 1 only when phosphorylated at Ser412. Does not crossreact with beta-arrestin 2. Species Crossreactivity: Mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Lefkowitz, R.J. (1998) J. Biol. Chem. 273, 18677–18680. 2. Lin, F. et al. (1999) J. Biol. Chem. 274, 15971–15974. 3. Lin, F. et al. (1997) J. Biol. Chem. 272, 31051–31057.