Technical Data
Arrestin 1, beta, phosphorylated (Ser412) (ARRB1, ARB1, ARR1)
Beta-arrestin 1 is a member of the arrestin family, which has been implicated in mediating the desensitization and internalization of G protein-coupled receptors (GPCR). Beta-arrestin 1 is constitutively phosphorylated by Erk1/2 at serine 412 of the C-terminus and localized in the cytosol. When recruited to the plasma membrane after agonist stimulation of the beta2-adrenergic receptors, beta-arrestin 1 is rapidly dephosphorylated. This dephosphorylation is an essential step of beta-arrestin 1-mediated receptor endocytosis, but it is not required for receptor desensitization.

Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:100. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanMouse
Not Determined
Synthetic peptide corresponding to residues surrounding Ser412 of human beta-arrestin 1.
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.
Recognizes endogenous human beta-arrestin 1 only when phosphorylated at Ser412. Does not crossreact with beta-arrestin 2. Species Crossreactivity: mouse, rat and monkey
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lefkowitz, R.J. (1998) J. Biol. Chem. 273, 1867718680. 2. Lin, F. et al. (1999) J. Biol. Chem. 274, 1597115974. 3. Lin, F. et al. (1997) J. Biol. Chem. 272, 3105131057.