Technical Data
Arrestin 1, beta, phosphorylated (Ser412) (ARRB1, ARB1, ARR1)
-arrestin-1 is a member of the arrestin family that regulates beta-adrenergic receptor function. Beta-arrestins seem to mediate the desensitization and internalization of G protein-coupled receptors (1). -arrestin-1 is phosphorylated by Erk1/2 at Serine 412 of the C-terminus (2). Expanded roles for -arrestin-1 have been studied. Interaction with molecules such as clathrin, AP-2 and NSF directs the clathrin-mediated internalization of G-protein-coupled receptors. Interaction with molecules such as Src, Raf, Erk, ASK1 and JNK3 appears to regulate several pathways that result in the activation of MAP kinases (3,4).

Suitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blotting: 1:20,000
Immunohistochemistry: 1:500
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:100

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20CBlue IceHumanRabbit
A synthetic phospho-peptide corresponding to residues surrounding Ser412 of human-B-Arrestin-1
Supplied as a liquid
Recognizes human-B-Arrestin-1 when phosphorylated at Ser412.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lefkowitz, R.J. G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization. J. Biol. Chem. 273: 1867718680 (1998). 2. Lin, F.T., et al. Feedback regulation of beta-arrestin1 function by extracellular signal-regulated kinases. J. Biol. Chem. 274: 1597115974 (1999). 3. Lin, F.T., et al. beta-arrestins regulate mitogenic signaling and clathrin-mediated endocytosis of the insulin-like growth factor I receptor. J Biol Chem. 273: 316403 (1998). 4. Miller, W.E., and R.J. Lefkowitz. Expanding roles for beta-arrestins as scaffolds and adapters in GPCR signaling and trafficking. Curr Opin Cell Biol. 13: 13945 (2001).