Technical Data
A3577-01D
Arrestin 1, beta, phosphorylated (Ser412) (ARRB1, ARB1, ARR1)
Description:
ß-arrestin-1 is a member of the arrestin family that regulates beta-adrenergic receptor function. Beta-arrestins seem to mediate the desensitization and internalization of G protein-coupled receptors (1). ß-arrestin-1 is phosphorylated by Erk1/2 at Serine 412 of the C-terminus (2). Expanded roles for ß-arrestin-1 have been studied. Interaction with molecules such as clathrin, AP-2 and NSF directs the clathrin-mediated internalization of G-protein-coupled receptors. Interaction with molecules such as Src, Raf, Erk, ASK1 and JNK3 appears to regulate several pathways that result in the activation of MAP kinases (3,4).

Applications:
Suitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blotting: 1:20,000
Immunohistochemistry: 1:500
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:100

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG6k28Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Ser412 of human-B-Arrestin-1
Purity:
Supernatant
Form
Supplied as a liquid
Specificity:
Recognizes human-B-Arrestin-1 when phosphorylated at Ser412.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lefkowitz, R.J. G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization. J. Biol. Chem. 273: 18677–18680 (1998). 2. Lin, F.T., et al. Feedback regulation of beta-arrestin1 function by extracellular signal-regulated kinases. J. Biol. Chem. 274: 15971–15974 (1999). 3. Lin, F.T., et al. beta-arrestins regulate mitogenic signaling and clathrin-mediated endocytosis of the insulin-like growth factor I receptor. J Biol Chem. 273: 31640–3 (1998). 4. Miller, W.E., and R.J. Lefkowitz. Expanding roles for beta-arrestins as scaffolds and adapters in GPCR signaling and trafficking. Curr Opin Cell Biol. 13: 139–45 (2001).