Technical Data
Arrestin 1, beta (ARRB1, ARB1, ARR1)
b-arrestin 1 (ARRB1, ARB1, ARR1) is a member of the arrestin protein family, which also includes visual arrestin, cone arrestin and b-arrestin 2. b-arrestins bind phosphorylated heptahelical G protein coupled receptors to desensitize and target them to clathrin-coated pits for endocytosis. b-arrestin 1 acts as a cofactor in the b-adrenergic receptor kinase (BARK)-mediated desensitization of b-adrenergic receptors, resulting in specific dampening of cellular responses to stimuli such as hormones, neurotransmitters or sensory signals (1). b-arrestin 1 exists as two alternately spliced transcripts, encoding two isoforms. Isoform A is a longer variant, while isoform B lacks an alternate in-frame exon compared to isoform A, resulting in a shorter protein (1). The functions of the isoforms have not been fully described. b-arrestin 1 is a cytosolic protein, and is widely expressed in cells, particularly in the central nervous system and in peripheral blood leukocytes (2).

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.1-1ug/ml
Western Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Controls:
Mouse RAW264.7 nuclear extracts, rat PC-12 cell lysates.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,k7G45Affinity Purified
100ug-20CBlue IceMouse
Synthetic peptide corresponding to the C-terminal region of human beta-arrestin 1, which shares 84% sequence homology with mouse and rat.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Reactivity has been confirmed with mouse RAW264.7nuclear extracts and rat PC-12 cell lysates. Based on amino acid sequence homology, reactivity with human is expected.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Parruti G, et al. J Biol Chem 268(13):9753-9761, 1993. 2. Kohout TA, et al. PNAS 98(4):1601-1606, 2001.
General References:
1. Lefkowitz, R.J. G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization. J. Biol. Chem. 273: 1867718680 (1998). 2. Lin, F.T., et al. Feedback regulation of beta-arrestin1 function by extracellular signal-regulated kinases. J. Biol. Chem. 274: 1597115974 (1999). 3. Lin, F.T., et al. beta-arrestins regulate mitogenic signaling and clathrin-mediated endocytosis of the insulin-like growth factor I receptor. J Biol Chem. 273: 316403 (1998). 4. Miller, W.E., and R.J. Lefkowitz. Expanding roles for beta-arrestins as scaffolds and adapters in GPCR signaling and trafficking. Curr Opin Cell Biol. 13: 13945 (2001).