Technical Data
A3577-01E
Arrestin 1, beta (ARRB1, ARB1, ARR1)
Description:
b-arrestin 1 (ARRB1, ARB1, ARR1) is a member of the arrestin protein family, which also includes visual arrestin, cone arrestin and b-arrestin 2. b-arrestins bind phosphorylated heptahelical G protein coupled receptors to desensitize and target them to clathrin-coated pits for endocytosis. b-arrestin 1 acts as a cofactor in the b-adrenergic receptor kinase (BARK)-mediated desensitization of b-adrenergic receptors, resulting in specific dampening of cellular responses to stimuli such as hormones, neurotransmitters or sensory signals (1). b-arrestin 1 exists as two alternately spliced transcripts, encoding two isoforms. Isoform A is a longer variant, while isoform B lacks an alternate in-frame exon compared to isoform A, resulting in a shorter protein (1). The functions of the isoforms have not been fully described. b-arrestin 1 is a cytosolic protein, and is widely expressed in cells, particularly in the central nervous system and in peripheral blood leukocytes (2).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.1-1ug/ml
Western Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Controls:
Mouse RAW264.7 nuclear extracts, rat PC-12 cell lysates.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG1,k7G45Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminal region of human beta-arrestin 1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the beta-arrestin 1 (ARRB1, ARB1, ARR1) protein (~55kD). Species Crossreactivity: mouse and rat. Species Sequence Homology: human, 84%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Parruti G, et al. J Biol Chem 268(13):9753-9761, 1993. 2. Kohout TA, et al. PNAS 98(4):1601-1606, 2001.
General References:
1. Lefkowitz, R.J. G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization. J. Biol. Chem. 273: 18677–18680 (1998). 2. Lin, F.T., et al. Feedback regulation of beta-arrestin1 function by extracellular signal-regulated kinases. J. Biol. Chem. 274: 15971–15974 (1999). 3. Lin, F.T., et al. beta-arrestins regulate mitogenic signaling and clathrin-mediated endocytosis of the insulin-like growth factor I receptor. J Biol Chem. 273: 31640–3 (1998). 4. Miller, W.E., and R.J. Lefkowitz. Expanding roles for beta-arrestins as scaffolds and adapters in GPCR signaling and trafficking. Curr Opin Cell Biol. 13: 139–45 (2001).