Technical Data
A3577-01H
Arrestin 1,2 beta (ARRB1, ARB1, ARR1, ARRB2, ARB2, ARR2)
Description:
Arrestin proteins function as negative regulators of G protein coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylat-ed GPCR and the eventual internalization of the receptor and
desensitization of GPCR signaling (1). Four distinct mam-malian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (or X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as Beta-arrestin 1) and arrestin 3 (or Beta-arrestin 2) are ubiqui-tously expressed and bind to most GPCRs (2). Beta-arrestin proteins function as adapters and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in ERK activation pathways (3,4). Beta arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that Beta-arrestin proteins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10E5Affinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues surrounding Phe386 of human SGK2 protein.tissues using SGK2 antibody.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 0.02% sodium azide and 50% glycerol.
Specificity:
Recongnizes endogenous levels of human total SGK2 protein. Species Crossreactivity: Rat, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shenoy, S.K. and Lefkowitz, R.J. (2005) Sci STKE 2005, cm10, 2. Lefkowitz, R.J. and Shenoy, S.K. (2005) Science 308, 512-7, 3. Luttrell, L.M. et al. (1999) Science 283, 655-61, 4. Luttrell, L.M. et al. (1999) Curr Opin Cell Biol 11, 177-83, 5. Luttrell, L.M. and Lefkowitz, R.J. (2002) J Cell Sci 115,
455-65, 6. Waters, C. et al. (2004) Semin Cell Dev Biol 15, 309-23, 7. Lefkowitz, R.J. and Whalen, E.J. (2004) Curr Opin Cell Biol 16, 162-8, 8 Waters, C.M. et al. (2005) Cell Signal 17, 263-77, 9. Kang, J. et al. (2005) Cell 123, 833-47, 10. Ma, L. and Pei, G. (2007) J Cell Sci 120, 213-8.