Technical Data
A3577-02E
ARRB2 (Beta-arrestin-2, Arrestin beta-2, ARB2, ARR2)
Description:
Beta-arrestin-2, or ARRB2, is an adaptor protein belonging to the arrestin family with an N- arrestin domain and a C- arrestin domain. ARRB2 is a regulatory protein involved in heterotrimeric G protein-coupled receptor desensitization and is known to regulate beta2-adrenergic receptor (beta2AR) function by enhancing beta2AR mediated nuclear translocation of ERK. ARRB2 binds to phosphorylated beta2ARs, thereby causing a significant impairment of their capacity to activate G(S) proteins. Along with AIP4, ARRB2 acts as an endosomal sorting molecule that mediates CXCR4 entry into a degradative pathway. It may also be involved in hormone-specific desensitization of TSH receptors. ARRB2 is a Ca2+ binding protein of the retinal outer segments and binds to P-rhodopsin and is also involved in synaptic transmission in photoreceptor cells. It is widely expressed in most of the tissues, especially neuronal tissues and spleen.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.25-0.5ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HUVEC cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa23-40 (GKRDFVDHLDKVDPVDG) of human ARRB2, Gen Bank no. gb|ABG47460.1|.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human ARRB2. Species Crossreactivity: bovine, canine, mouse, equine, monkey, rat. Species sequence homology: pufferfish, zebrafish, xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Alloway, PG. et al. Neuron. 28:129-138 (2000). 2. Attramadal, H. et al. J. Biol. Chem. 267:17882-17890 (1992). 3. Chen, W. et al. Science. 306:2257-2260 (2004). 4. Chen, W. et al. Science. 301:1394-1397 (2003).