Technical Data
ARRB2 (Beta-arrestin-2, Arrestin beta-2, ARB2, ARR2)
Beta-arrestin-2, or ARRB2, is an adaptor protein belonging to the arrestin family with an N- arrestin domain and a C- arrestin domain. ARRB2 is a regulatory protein involved in heterotrimeric G protein-coupled receptor desensitization and is known to regulate beta2-adrenergic receptor (beta2AR) function by enhancing beta2AR mediated nuclear translocation of ERK. ARRB2 binds to phosphorylated beta2ARs, thereby causing a significant impairment of their capacity to activate G(S) proteins. Along with AIP4, ARRB2 acts as an endosomal sorting molecule that mediates CXCR4 entry into a degradative pathway. It may also be involved in hormone-specific desensitization of TSH receptors. ARRB2 is a Ca2+ binding protein of the retinal outer segments and binds to P-rhodopsin and is also involved in synaptic transmission in photoreceptor cells. It is widely expressed in most of the tissues, especially neuronal tissues and spleen.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.25-0.5ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HUVEC cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa23-40 (GKRDFVDHLDKVDPVDG) of human ARRB2, Gen Bank no. gb|ABG47460.1|.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Recognizes human ARRB2. Species Crossreactivity: bovine, canine, mouse, equine, monkey, rat. Species sequence homology: pufferfish, zebrafish, xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Alloway, PG. et al. Neuron. 28:129-138 (2000). 2. Attramadal, H. et al. J. Biol. Chem. 267:17882-17890 (1992). 3. Chen, W. et al. Science. 306:2257-2260 (2004). 4. Chen, W. et al. Science. 301:1394-1397 (2003).