Technical Data
ART3, CT (Ecto-ADP-ribosyltransferase 3, NAD(P)(+)-arginine ADP-ribosyltransferase 3, Mono(ADP-ribosyl) Transferase 3, TMART)
Mono-ADP-ribosylation involves the transfer of the ADP-ribose moiety from NAD+ to a specific amino acid in the target protein. The rodent mono-ADP-ribosyltransferase RT6 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein specifically expressed at the cell surface of rat and mouse T lymphocytes. The predicted 367aa human ART3 protein has an estimated molecular mass of 41.5kD and contains a hydrophobic peptide signal at its N terminus, 3 consensus motifs specific to enzymes catalyzing ADP-ribose transfer, a hydrophobic C-terminal sequence characteristic of a GPI-anchored protein, a novel motif repeated 3 times at its C terminus, and 1 potential glycosylation site.1 The ART3 and rodent RT6 proteins share 35% amino acid identity.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
Synthetic peptide selected from the C-terminal region of human ART3 (KLH).
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human ART3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2.Koch-Nolte, F., et al., Genomics 39(3):370-376 (1997). 3.Levy, I., et al., FEBS Lett. 382(3):276-280 (1996).