Technical Data
Artemin, Recombinant, Human (GFRalpha3-RET Ligand)
Molecular Biology Storage: -20CShipping: RT
Recombinant Human Artemin is a disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2x113 amino acids.

Sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Gly-Gly-Pro-Gly.

Dimers and Aggregates:
1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:
Artemin is fully biologically active when compared to standard. The activity is calculated by it's ability to promote survival and neurite outgrowth and dorsal root ganglion neurons.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm
2. Analysis by RP-HPLC, using a calibrated solution of Artemin as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Recombinant, Human (E. coli)
Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: 0.1ng/ug (IEU/ug)
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder in 10mM sodium citrate, pH 4.5, 150mM sodium chloride. Reconstitute the lyophilized Human Artemin in sterile ddH2O, 0.1% HSA or BSA to 100ug/ml, which can then be further diluted to other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Lucini, C., et al., Anat. Embryol. (Berl) 208(5): 403-410 (2004). 2. Lucini, C., et al., Anat. Embryol. (Berl) Jul 28 (2004). 3. Bolon, B., et al., Toxicol. Pathol. 32(3): 275-294 (2004). 4. Warner, A.H., et al., Arch. Biochem. Biophys. 424(2): 189-200 (2004). 5. Gardell, L.R., et al., Nat. Med. 9(11): 1383-1389 (2003). 6. Yan, H. and Newgreen, D.F., Dev. Dyn. 227(3): 395-401 (2003).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.