Technical Data
A3585-02A
Artemis (DCLRE1C, Protein Artemis, DNA Cross-link Repair 1C Protein, Protein A-SCID, SNM1 Homolog C, hSNM1C, SNM1-like Protein, ASCID, SCIDA, SNM1C)
Description:
Artemis has a role in T and B lymphocyte immunodeficiency and in predisposition to lymphoma through the NHEJ pathway of DNA repair. DNA-PKcs regulates Artemis by both phosphorylation and complex formation to permit enzymatic activities that are critical for the hairpin-opening step of V(D)J recombination and for the 5' and 3' overhang processing in non-homologous DNA end joining.

Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000
ELISA: 1:10,000-1:20,000
Immunohistochemistry (Formalin fixed paraffin embedded): 1.25-2.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa482-495 of human DCLRE1C (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity:
Recognizes human DCLRE1C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.