Technical Data
ARTS, Blocking Peptide (Apoptosis Related Protein in TGF b eta Signaling Pathway)
Molecular Biology Storage: -20CShipping: Blue Ice
Synthetic peptide (KRFLEDTTDDGE) corresponding to amino acids 3 to 14 of human ARTS (1).

Apoptosis is related to many diseases and development. Mitochondrial proteins, such as cytochrome c, Apaf-1, and AIF play important role in apoptosis. A novel mitochondrial septin-like protein was identi ed recently and designated ARTS for apoptosis related protein in TGF-b signaling pathway (1). ARTS that is encoded by the human septin H5/PNUTL2/CDCrel2b gene (1-4) is located to mitochondria and translocates to the nucleus when apoptosis occurs. ARTS is expressed in many tissues. It enhances cell death induced by TGF-b and, to a lesser extent, by other apoptotic agents, such as TNF-a and Fas ligand.

Applications: Suitable for use in ELISA and Western Blot. Other applications not tested.

Storage and Stability: Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile 25% glycerol and store at -20C. May be stored at 4C for short-term only. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Synthetic peptide
Purity: Purified 60-70%
Concentration: ~0.2mg/ml
Form: Supplied as a lyophilized powder.

Intended for research use only, not for use in human, therapeutic or diagnostic applications.
1. Larisch S, Yi Y, Lotan R, Kerner H, et al. A novel mitochondrial septin-like protein, ARTS, mediates apoptosis dependent on its P-loop motif. Nat Cell Biol 2000;2(12):91521.
2. Depraetere V. The ARTS of apoptosis. Nat Cell Biol. 2000;2(12):E219.
3. Paavola,P., Horelli-Kuitunen,N., Palotie,A. and Peltonen,L. Characterization of a novel gene, PNUTL2, on human chromosome 17q22-q23 and its exclusion as the Meckel syndrome gene. Genomics 1999;55 (1):122125.
4. Zieger,B., Tran,H., Hainmann,I., Wunderle,D., Zgaga-Griesz,A., Blaser,S. and Ware,J. Characterization and expression analysis of two human septin genes, PNUTL1 and PNUTL2. Gene 2000;261(2):197203.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.