Technical Data
ASC (Apoptosis-associated Speck-like Protein Containing a CARD, hASC, Caspase Recruitment Domain-containing Protein 5, PYD and CARD Domain-containing Protein, Target of Methylation-induced Silencing 1, PYCARD, CARD5, TMS1)
Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells. Overexpression of ASC induced DNA fragmentation. ASC is expressed in a variety of human and mouse tissues.

Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunocytochemistry: 5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HL60 whole cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa near the C-terminus of human ASC.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Recognizes human ASC.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Masumoto, J. et al. J. Biol. Chem; 274, 33835 (1999). 2. Masumoto, J. et al. Exp. Cell Res. 262, 128 (2001). 3. Conway, KE. et al. Cancer Res. 60, 6236 (2000). 4. McConnell, BB. et al. Cancer Res. 60, 6243 (2000).