Technical Data
Ascorbate Oxidase (AO, Ascorbase, ASO, L-ascorbate Oxidase Precursor)
Ascorbate Oxidase is a copper-containing enzyme. It catalyzes the following reaction:
Ascorbic acid + O2 -----> Dehydroascorbic acid + H2O.
Ascorbate Oxidase is widely distributed and is only present in plants. Its biological role in plants is still not clear. Studies after increasing and reducing the enzyme activity suggest that it has a positive effect on plant growth. In addition, it has been proposed that the enzyme may be involved in plant defense by modifying the ascorbate level and thus redox state in apoplast.

Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:25,000-1:125,000
Western Blot:1:2000-1:10,000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGHighly Purified
100ug-20CBlue IceRabbit
~1mg/ml (after reconstitution)
Ascorbate Oxidase (cucurbita).
Purified by Ion Exchange chromatography.
Supplied as a lyophilized powder from 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with 100ul sterile ddH2O.
Recognizes Ascorbate Oxidase from Cucurbita. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum as well as purified and partially purified Ascorbate Oxidase (cucurbita).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.